|Nicholson holds regenerated Pixie grape plants. Photo by Brian Charles Clark|
PULLMAN, Wash. – Understanding the grape genome in all its vast variety will translate into sustainable viticulture practices and a deeper understanding of wine quality.
Getting a grip on grape genetics requires not just the sequencing of entire genomes but detailed pictures of which genes do what. To get that information, scientists need a way to quickly grow sample plants that have been genetically transformed. Transformation means that a particular gene is silenced or added to the organism in order to learn what effect the change has on the plant.
|Amit Dhingra on Pixie grape research at WSU|
“We need regeneration in order to do transformation,” said Kathie Nicholson, a doctoral student working in horticultural genomicist Amit Dhingra’s lab. “But it’s been hard to find a reliable method of regenerating grape in the lab.” Regeneration is a carefully controlled laboratory technique in which whole plants are grown from a few cells.
|Nathan Tarlyn, one of Nicholson’s co-authors, working on a plant tissue culturing project. Photo by Brian Charles Clark/WSU.|
“Technically, traditional breeding is genetic transformation,” Nicholson said, but traditional breeding is like taking a slow boat to an uncertain shore: you don’t know when you’re going to get there and you can never be sure where you are going. With the systematic exploration of gene function, Nicholson said, researchers will be able to zero in on the precise factors that give a plant resistance to certain diseases, the ability to withstand stresses like cold and drought, and the ability to produce the combination of complex chemicals that are so prized by winemakers.
Granted, Nicholson said, Pixie may not be an ideal model for studying wine grape genetics (although Pixie was developed from Pinot Meunier, an important grape used in the production of champagne.) “That’s why we are trying the same regeneration protocol on Chardonnay and Merlot,” she said.
Plants have the ability to regenerate themselves from a single cell. This ability, called totipotency, is sometimes compared to the ability of animal stem cells (which are pluripotent) to differentiate themselves into the wide variety of cells in an organism. A plant cell can divide, differentiate, and become the cells of shoots, roots, and leaves. This, anyway, is the theory: getting a few plant cells to differentiate and grow into mature plants in the lab is much more difficult.
Nicholson and her colleagues experimented with a variety of mixes of plant growth hormones and regimes of light and dark in order to find the sweet spot that encouraged the regeneration of Pixie. Starting with a tiny piece of leaf from the very top of a plant, she and her team found that a particular combination of stress-easing dark, a particular ratio of hormones, and a nutrient medium coaxed the cells to regrow into new Pixie plants.
This article is based in part on a paper by Nicholson et al. in Plant Cell, Tissue and Organ Culture and available online at http://bit.ly/Q5JyZ6.